Fig. 1
From: Intracellular targeting of Cisd2/Miner1 to the endoplasmic reticulum
The N-terminal portion of Cisd2 targets it to the ER. a Schematic representation of natural and chimeric Cisd1 and Cisd2 proteins. The Cisd12 (respectively Cisd21) protein is composed of the N-terminal portion of Cisd1 (respectively Cisd2) attached to the Cisd2 (respectively Cisd1) cytosolic domain. The detailed amino acid sequence of these proteins is shown in Additional file 4. b Immunofluorescence localization of Cisd proteins. HEK cells were co-transfected to produce the indicated Cisd protein, ER-targeted YFP and mitochondria-targeted RFP. Immunofluorescence staining was performed using specific antibodies against Cisd1 or Cisd2 cytoplasmic domain. All pictures were taken with a confocal microscope (LSM700, Zeiss). Scale bar: 10 μm. The arrowheads point to aggregates of mitochondria. A second panel of pictures is shown in Additional file 5