Fig. 1

An HG diet elevates cleaved caspase 3 levels and induces activation of Fyn and mTOR/S6K in the substantia nigra of rats. Cleaved caspase 3 levels in the substantia nigra of the HG (40 days) group were significantly higher than those in the control group (n = 5). The grouping of blots cropped from different parts of the same gel is shown. (B) Rat brain sections were stained with cleaved caspase 3 antibody (red) and tyrosine hydroxylase (TH) antibody (green). (C) Brain sections were stained with TUNEL (red) and an antibody against TH (green). (D) Rat brain sections were stained with antibodies against Fyn or mTOR antibody (red) and TH (green). (E) p-Y416 levels in the substantia nigra were elevated in the HG group compared to the control group (n = 5), but Fyn levels were not influenced. The blots of β-actin and Fyn were cropped from different parts of the same gel, and p-Y416 was cropped from different gels. (F) qRT–PCR indicated that mRNA levels of Fyn were unchanged by HG (n = 5). (G) p-mTOR at Ser2448 and p-S6K at Thr389 in the HG group were significantly higher than those in the control group, but total mTOR and S6K levels were comparable (n = 5). β-actin and S6K were cropped from different parts of the same gel, and p-S6K, mTOR and p-mTOR were cropped from different gels