Fig. 3

Compression induced calcium signaling in MDA-MB-231 cells. Representative images of intracellular [Ca²⁺] (a and c, bar = 100 μm) visualized by confocal microscopy (60X) and time-courses of changing relative mean fluorescence intensity (b and d) of Fluo-4 or G-GECO (normalized to time 0) in MDA-MB-231 cells labeled with Fluo-4/AM or transiently expressing G-GECO before (0 min) and after (1 min) exposure to compression at 200, 400, 600 Pa, respectively. e, f Time-courses of changing relative mean fluorescence intensity of G-GECO in MDA-MB-231 cells pretreated with or without EGTA, Gd³⁺, GsMTx4, and Piezo1 KD in response to 400 Pa compression. Each experiment assayed 10–20 cells and repeated three times. Black bars in b, d, e, f indicate the period of compression