Fig. 5

Compression enhanced the activity of Src and ERK. a Western blot analyses of the phosphorylation of Src and ERK in MDA-MB-231 cells pretreated with scramble probes in the absence or presence of compression at 200, 400, 600 Pa. Cropped images of Western blot are shown and uncropped images are shown in Fig. S8d-g. b Western blot analyses of the phosphorylation of Src and ERK in MDA-MB-231 cells pretreated with siRNA for Piezo1 in the absence or presence of compression at 200, 400, 600 Pa. Cropped images of Western blot are shown and uncropped images are shown in Fig. S8h-k. Relative phosphorylation levels were obtained by normalizing to GAPDH expression and value in control (Ctr) groups, n = 3. ^*p < 0.05 versus Ctr groups; ^** p < 0.01 versus Ctr groups. c Quantification of fold change of invaded cells in 400 Pa compression to the Ctr group pretreated with DMSO (vehicle). Data are presented as means ± s.e.m., n = 3, ^** p < 0.01 versus Ctr groups