Fig. 3

ErbB3 interacts with the nucleolar proteins UBF and C23. The expression of 47S pre-rRNA precursor in HeLa and U-87MG cells was analyzed by qPCR before and after NRG1 treatment (A). HeLa cells were immunostained with anti ErbB3 (green) and anti UBF (red). The colocalization is shown in yellow (B). Scalebar represent 5 μm. Nuclear extracts from HeLa and U-87MG cells were immunoprecipitated with anti ErbB3 and analyzed by western blot with anti UBF (C, D) and anti C23 antibodies (C). The heavy chains are shown as well. The nuclear localization of C23 was investigate by western blot in nuclear extracts before and after neuregulin1 treatment (E). The densitometric quantification of the C23 signal was performed by Image J (E, right) . Data are the average of three independent experiments and the significant results are highlighted with asterisks (* p ≤ 0.05)