Fig. 5

ErbB3 silencing affects cell cycle progression and cell survival. U-87MG (A-C) cells were transfected with 30 nM of siErbB3 or siScramble as control. The efficiency of ErbB3 silencing was analysed at mRNA level, by qPCR (A) and at protein level by western blot (B). The densitometric quantification of ErbB3 signal, in western blots, was performed by using the Image J software (C). U-87MG cells, transfected with siErbB3 at 30 nM for 24 h, show a decrease percentage of cells in S phase after cytofluorimetry analysis (D). U-87MG cell viability was analysed by trypan blue after 24 and 72 h of treatment with 30 nM of siErbb3 (E). Representative images of untreated cells and cells treated with 30 nM of siErbB3 or siScramble are shown (F). Scalebar represent 100 μm. Data are the mean of three independent experiments. Significant results are highlighted with asterisks (** p < 0.01, *** p < 0.001)