Fig. 7
Transcriptome (mRNAs) expression profiles of spheroids. (A) Quantitative analysis of differentially expressed genes grouped to its coding or noncoding function (fold change ≥. 2.0, p-value ≤0.05). (B) Number of differentially expressed protein coding genes (fold change ≥. 2.0, p-value ≤0.05). (C) Transcriptome (mRNAs) expression profiles of spheroids AIREWT compared to AIRE−/−. The spheroid cells’ total RNA samples were analyzed through RNA-seq, which allowed identify the differentially expressed mRNAs. The dendrograms and unsupervised heat-maps were obtained using the R platform, the cluster and tree view algorithm considering 2.0-fold-change and 0.05 false discovery rate. Heat-map legend: red = upregulated, blue = downregulated (Pearson’s correlation metrics)