Fig. 4

MAPKAPK5-AS1 played a role in HCC by regulating miR-429 expression. A The binding site of MAPKAPK5-AS1 and miR-429. B Luciferase activity was examined in 293 T cells transfected with MAPKAPK5-AS1-WT or MAPKAPK5-AS1-MUT and miR-3429 mimic or miR-NC. C Spearman correlation analysis of MAPKAPK5-AS1 expression and miR-429 expression in HCC tissues. D qRT-PCR was used to detect the expression of miR-429 in HCC cells after the cells were transfected with si-MAPKAPK5-AS1 or si-NC. E qRT-PCR was used to detect the expression of miR-429 after HCC cells were transfected with miR-429 inhibitors or miR-NC. F-I MTT and Transwell assays were adopted to detect the HCC cell proliferation, migration and invasion. J Flow cytometry was adopted to detect the HCC cell apoptosis. K Western blot was used to measure E-cadherin and N-cadherin expressions in HepG2 and Huh7 cells. *P < 0.05; **P < 0.01; ***P < 0.001