Fig. 4

OSGIN2 regulates jawbone BMSCs osteogenesis under oxidative stress via RORα/BSP-OCN signaling. A Expression of RORα mRNA was detected using qPCR after treatment of H₂O₂ and anti-OSGIN2 (Control as control group: no H₂O₂, siOSGIN2 or negative control of siOSGIN2 treatment). B Expressions of BSP and OCN mRNAs were detected using qPCR, C Mineralized nodules were detected using alizarin red, D Ectopic bone formations were detected using H&E after treatment of H₂O₂, anti-OSGIN2 and anti-RORα (Control (H₂O₂) as control group: H₂O₂ treatment, but no siOSGIN2, siRORα or negative control of siRORα treatment). n = 5. Scale bar: 500 μm. *p < 0.05, **p < 0.01, ***p < 0.001