Fig. 3

CCT3 promotes the growth and cell cycle progression of LUAD cells. A H1975 cells were infected with Ctrl and CCT3 overexpression lentivirus. qRT-PCR and Western blot analysis of CCT3 in Ctrl and CCT3 overexpressed H1975 cells. GAPDH is the internal control. **p < 0.01. The blots were cut prior to hybridisation with antibodies during blotting. B Ctrl and CCT3 overexpressed H1975 cells were subjected to CCK8 analysis of cell proliferation. *p < 0.05. C Colony formation analysis was performed on Ctrl and CCT3 overexpressed H1975 cells. *p < 0.05. D qRT-PCR analysis of Ki-67 and PCNA in Ctrl and CCT3 overexpressed H1975 cells. GAPDH is the internal control. *p < 0.05. E H2228 cells were infected shCtrl, shCCT3#1 and shCCT3#2 knockdown lentivirus. qRT-PCR and Western blot analysis of CCT3 in shCtrl, shCCT3#1 and shCCT3#2 H2228 cells. GAPDH is the internal control. *p < 0.05. The blots were cut prior to hybridisation with antibodies during blotting. F shCtrl, shCCT3#1 and shCCT3#2 H2228 cells were subjected to CCK8 analysis of cell proliferation. *p < 0.05. G Colony formation analysis was performed on shCtrl, shCCT3#1 and shCCT3#2 H2228 cells. *p < 0.05. H qRT-PCR analysis of Ki-67 and PCNA in shCtrl, shCCT3#1 and shCCT3#2 H2228 cells. GAPDH is the internal control. *p < 0.05. I Cell cycle was analyzed by PI staining in Ctrl and CCT3 overexpressed H1975 cells. Left, representative images of cell cycle. Right, quantification of cell cycle. *p < 0.05. J qRT-PCR analysis of cyclin D1 in Ctrl and CCT3 overexpressed H1975 cells. GAPDH is the internal control. **p < 0.01. K Cell cycle was analyzed by PI staining in shCtrl, shCCT3#1 and shCCT3#2 H2228 cells. Left, representative images of cell cycle. Right, quantification of cell cycle. *p < 0.05. J qRT-PCR analysis of cyclin D1 in shCtrl, shCCT3#1 and shCCT3#2 H2228 cells. GAPDH is the internal control. **p < 0.01