Fig. 1

Macrophage conditioned media induce formation of TNTs in PANC-1 cells. a Typical morphology of PANC-1 cells in DMEM, macrophage CMs, and 200 ng/ml EGF respectively. b Average numbers of TNTs/cell in PANC-1 cells under the treatments of macrophage CMs and 200 ng/ml EGF. ***, P < 0.005 in comparison with the PACN-1 cells in DMEM (post hoc Tukey’s test). c Typical SEM image of PANC-1 cells cultured in M0 CM. d, e Confocal fluorescence images of cytoskeletons (green, microtubules; orange, F-actin) in PANC-1 cells in DMEM and M0 CM, respectively. The blue color represents DAPI-stained nuclei