Fig. 4

Mitochondria and lysosomes can be transported between PANC-1 cells in M0 CM connected by a TNT. a Overlapped bright-field and fluorescence images showing two PANC-1 cells connected by a TNT. b–f Time-lapse confocal fluorescence images of mitochondrial trafficking within the TNT in (a). The two PANC-1 cells were sub-cultured and labeled with MitoTracker™ Green FM and MitoTracker™ Orange CMTMR, individually. Two mitochondria from each cell moved forward in opposite directions, collided, and then moved together into the upper cell. g Fluorescence image of lysosomes in PANC-1 cells. The lysosomes were labeled with LysoTracker® Green. h Magnified view of the area enclosed by a red dashed square in (g)