Fig. 3

MiR-145-5p represses the proliferation, migration, and invasion of nasopharyngeal carcinoma (NPC) cell lines. a A schematic illustration describing that putative miR-143-3p, miR-148-3p, miR-145-5p, miR-153-3p, miR-21-5p and miR-214-5p  might directly target KLF5. Data was obtained and analyzed from four prediction databases: TargetScan, miRDB, mirDIP, and miRanda. b Real-time PCR results showing the expression of miR-145-5p in NP69 and four NPC cell lines. c Real-time PCR results indicating miR-145-5p expression in four NPC cells transfected with scramble control or miR-145-5p mimics. d Proliferation rates of NPC cells 24 to 72 h post-transfection with a scramble control or miR-145-5p mimics. e Proliferation rates of NPC cells at 72 h post-transfection with a scramble control or miR-145-5p mimics was analyzed by BrdU ELISA cell proliferation assay. f NPC cells were transiently transfected with scramble control and miR-145-5p mimics and analyzed by wound healing assays. g NPC cells were transiently transfected with scramble control and miR-145-5p mimics and analyzed by invasion assays. h Proliferation rates of NP69 cells at 72 h post-transfection with a scramble control or miR-145-5p mimics was analyzed by BrdU ELISA cell proliferation assay. i NP69 cells were transiently transfected with scramble control and miR-145-5p mimics and analyzed by wound healing assays. j NP69 cells were transiently transfected with scramble control and miR-145-5p mimics and analyzed by invasion assays. Data are shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, respectively