Fig. 2

Establishment of iPSCs from Prg4-mRFP1 transgenic mice. a Quantitative RT-PCR analysis of pluripotency-related genes in embryonic stem cells (ESCs), ear tip fibroblasts (fibro), and six iPSC clones. The expression level of ESCs was set to 1. Data are presented as the mean of three technical replicates. b Bright-field image of iPSCs (#2–9). Scale bar: 200 μm. c Teratomas derived from iPSCs (#2–9) comprising ectodermal, mesodermal, and endodermal tissue. Scale bars: 100 μm