Fig. 1

Construction of a COVID-19-type proinflammatory model. A. Cell viability was assessed by a CCK-8 assay at 12 and 24 h. B-E. qRT‒PCR detection of the mRNA expression of ACE2, AGTR1, TMRRSS2, and ISG15 at 12 and 24 h. F-J. Western blot detection of the protein expression of ACE2, AGTR1, TMRRSS2, and ISG15 at 12 and 24 h. K-L. ELISAs were used to measure IL-6 and MCP-1 levels. The experiments were repeated three times. The significance of three or more groups was compared by one-way ANOVA. Tukey’s test was adopted for postmortem testing. * P < 0.05 vs. control, # P < 0.05 vs. RBD-500.