Fig. 2

The role of GH/E2/Tes in a COVID-19-type proinflammatory model

BEAS-2B cells were treated with 1000 ng/mL RBD and 200 ng/mL GH, 1 nM E2, or 10 nM Tes simultaneously for 24 h. A. CCK-8 assay for cell viability. B-E. qRT‒PCR detection of the mRNA expression of ACE2, AGTR1, TMRRSS2, and ISG15. F-J. Western blot detection of ACE2, AGTR1, TMRRSS2, and ISG15 protein expression. The experiments were repeated three times. The significance of three or more groups was compared by one-way ANOVA. Tukey’s test was adopted for postmortem testing. * P < 0.05 vs. control, # P < 0.05 vs. RBD, ns: no significance