Fig. 4
From: CARD9 contributes to ovarian cancer cell proliferation, cycle arrest, and cisplatin sensitivity
Association between HOXB5 and CARD9. A According to the GEPIA database, the level of HOXB5 in ovarian cancer was analyzed. B HOXB5 is positively correlated with CARD9 according to Spearman correlation. C The level of HOXB5 in ovarian cancer cell lines was detected by immunoblotting and (D) RT-qPCR. E The efficacy of HOXB5 overexpression and knockdown in SKOV3 cells were assessed by RT-qPCR and (F) immunoblotting. G HOXB5 overexpression could lead to increased levels of CARD9 from RT-qPCR and (H) immunoblotting results. I According to the JASPAR database, there is a binding site for the transcription factor HOXB5 in the promoter region of CARD9. J The association between CARD9 and HOXB5 was verified using dual-luciferase reporter assay and (K) ChIP. ***P < 0.001 vs. oe-NC or IOSE-80; ##P < 0.01, ###P < 0.001 vs. sh-NC