Fig. 7
From: RUNX3 mediates keloid fibroblast proliferation through deacetylation of EZH2 by SIRT1
Effect of SIRT1 on EZH2 acetylation levels in keloid fibroblasts. A EZH2 mRNA by RT-PCR. B-C Effect of SIRT inhibitor EX527 on EZH2 Acetylation Level. ##P < 0.01 vs Control. D-E Effect of CHX on protein stability was detected with Western blot. Representative (D) and quantitative (E) results were shown. *P < 0.05 vs 0 h, **P < 0.01vs 0 h, ***P < 0.001 vs 0 h. F-G Expression of protein stability in human keloid fibroblasts treated with SIRT1 inhibitor EX527. Representative (F) and quantitative (G) results were shown. Original blots/gels are presented in Supplementary Fig. 5 and 6