Fig. 1

Viability and cytotoxicity of doxorubicin- and 8-hydroxydeoxyguanosine-treated H9c2 cells. An MTT assay was used to examine H9c2 cell viability after treatment with various concentrations of DOX (A) and 8-OHdG (B) for 24, 48, and 72 h. C, After H9c2 cells were treated with DOX (1 μM) and 8-OHdG (50, 100, 250, and 500 μg/mL) for 24 h, cell proliferation and cytotoxicity were measured using MTT (C) and LDH (D) assays, respectively. DOX, doxorubicin; 8-OHdG, 8-hydroxydeoxyguanosine. ^**P < 0.01, ^***P < 0.001, and ^****P < 0.0001 versus control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, and ^####P < 0.001 versus DOX