Fig. 3

Exogenous 8-OHdG decreases NOX1/2/4 expression and NF-κB phosphorylation, and increases the reduced glutathione/oxidized glutathione ratio in DOX-treated H9c2 cells. A Rac1 activation assay. After H9c2 cells were treated with DOX (1 μM) and 8-OHdG (100 or 250 μg/mL) for 1 h, cell lysates were precipitated by p21-activated protein kinase (PAK) p21-binding domain (PBD) agarose beads and immunoblotted by Rac1 specific monoclonal antibody. B–D H9c2 cells were treated with 1 μM DOX and 100 μg/mL or 250 μg/mL 8-OHdG for 24 h. B–C Western blot analysis of the NOX1/2/4, p65, and phosphor-p65 protein levels of in DOX- and 8-OHdG-treated H9c2 cells. D GSH/GSSG ratio was determined using a glutathione assay kit. CTL, control; DOX, doxorubicin; 8-OHdG, 8-hydroxydeoxyguanosine; NOX, NADPH oxidase; GSH/GSSG, reduced glutathione/oxidized glutathione ratio. ^*P < 0.05 versus control; ^#P < 0.05 and ^##P < 0.01 versus DOX-treated groups