Fig. 4

Exogenous 8-OHdG decreases the expression of NLRP3, ASC, and caspase-1 in DOX-treated H9c2 cells. A-B H9c2 cells were treated with 1 μM DOX and 100 μg/mL or 250 μg/mL 8-OHdG for 24 h. A Quantitative real-time PCR for TNF-α, TLR2, TLR4, and NLRP3 in DOX- and 8-OHdG-treated H9c2 cells. B Western blot analysis ASC and caspase-1 protein levels in DOX- and 8-OHdG-treated H9c2 cells. CTL, control; DOX, doxorubicin; 8-OHdG, 8-hydroxydeoxyguanosine; TNF, tumor necrosis factor; TLR, toll-like receptor; NLRP, NLR family pyrin domain containing; ASC, apoptosis-associated speck-like protein containing a CARD c-terminal caspase recruitment domain. ^**P < 0.01 and ^****P < 0.0001 versus control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, and ^####P < 0.0001 versus DOX-treated groups