Exogenous 8-hydroxydeoxyguanosine attenuates doxorubicin-induced cardiotoxicity by decreasing pyroptosis in H9c2 cardiomyocytes

Table 1 Nucleotide sequences of qRT-PCR primers

PCR targets	Forward primer (5’–3’)	Reverse primer (5’–3’)
TNF-α	CATCCGTTCTCTACCCAGCC	AATTCTGAGCCCGGAGTTGG
IL-1β	TGCTGTCTGACCCATGTGAG	GTCGTTGCTTGTCTCTCCTTG
NLRP3	GTGGAGATCCTAGGTTTCTCTG	CAGGATCTCATTCTCTTGGATC
ASC	CTCTGTATGGCAATGTGCTGAC	GAACAAGTTCTTGCAGGTCAG
Caspase-1	GAGCTGATGTTGACCTCAGAG	CTGTCAGAAGTCTTGTGCTCTG
ANP	AAATCCCGTATACAGTGCGG	GGAGGCATGACCTCATCTTC
BNP	CCAGAACAATCCACGATGC	TCGAAGTCTCTCCTGGATCC
GATA4	CCCCAATCTCGATATGTTTGATGAC	GGGCCGGTTGATACCATTCA
GATA6	TGAACGGGACGTACCACCACCACC	ACAGTTCACGCACTCGCGGCTCTC
TLR2	GGCCACAGGACTCAAGAGCA	AGAGGCCTATCACAGCCATCAAG
TLR4	GGACTCTGCCCTGCCACCATTTA	CTTGTGCCCTGTGAGGTCGTTGA
GAPDH	GGCTCTCTGCTCCTCCCTGTTCTA	TGCCGTTGAACTTGCCGTGGG

TNF Tumor necrosis factor, IL Interleukin, NLRP3 NLR family pyrin domain containing 3, ASC Apoptosis-associated speck-like protein containing a c-terminal caspase recruitment domain, ANP Atrial natriuretic peptide, BNP Brain natriuretic peptide, TLR Toll-like receptor, GAPDH Glyceraldehyde 3-phosphate dehydrogenase

ISSN: 2661-8850