Fig. 5

SNX5 is a target of miR-520c-3p. A The role of miR-520c-3p on cell luciferase activity of SNX5-WT and SNX5-MUT reporter systems was measured by luciferase reporter assay. B, C The expression levels of SNX5 in OSCC tissues and cells were detected by RT-qPCR. D The knockdown level of SNX5 was confirmed in SCC-25 and CAL-27 cells by RT-qPCR. SCC-25 and CAL-27 cells were respectively transfected with si-NC plus in-NC, si-NC plus miR-520c-3p inhibitor, si-SNX5 plus in-NC, or si-SNX5 plus miR-520c-3p inhibitor, E cell proliferation was detected by CCK8 assay, F cell migration and invasion abilities were detected by transwell assays. G The SNX5 protein expression was explored in OSCC tissues and their adjacent normal tissues by western blot. H The effect of overexpression of HOXA-AS2 or miR-520c-3p on SNX5 protein expression was evaluated by western blot. I SCC-25 and CAL-27 cells were respectively transfected with si-NC plus in-NC, si-HOXA-AS2#1 plus in-NC, or si-HOXA-AS2#1 plus miR-520c-3p inhibitor, then SNX5 protein expression was detected by western blot. The experiment was repeated at least three times. *P < 0.05, **P < 0.01, and ***P < 0.001