Fig. 6

HOXA-AS2 regulates autophagy-mediated apoptosis through SNX5. A, B SCC-25 and CAL-27 cells were transfected with si-NC, si-HOXA-AS2#1, pcDNA, or HOXA-AS2, cell apoptosis was evaluated using TUNEL assay. Western blot was used to detect the protein expression levels of p-mTOR, mTOR, p62, LC3B, Bcl2, Bax in SCC-25 and CAL-27 cells with different transfections or treatment. C SCC-25 and CAL-27 cells were transfected with si-NC or si-SNX5. D SCC-25 and CAL-27 cells were transfected with si-NC, si-SNX5, or si-SNX5 plus treatment of 3MA. E SCC-25 and CAL-27 cells were transfected with pcDNA, HOXA-AS2, or HOXA-AS2 plus si-SNX5. F SCC-25 and CAL-27 cells were transfected with pcDNA, HOXA-AS2, or HOXA-AS2 plus miR-520c-3p mimic. The experiment was repeated at least three times. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with si-NC or pcDNA; #P < 0.05, ##P < 0.01 and ###P < 0.001 compared with HOXA-AS2