Fig. 4

Ceramide-induced activation of the TXNIP/NLRP3/GSDMD pathway contributes to HUVECs’ pyroptosis. HUVECs were pre-treated with MCC950 (10uM) for 2 h followed by the addition of C8-ceramide(20 μM) for a further 12 hours. Effects of MCC950 on the expression of IL-1β (A) and IL-18 (B). Values were reported as the mean ± standard deviation in (A–B) (n = 3). ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 vs control group. ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs ceramide treatment group