Fig. 6

TXNIP siRNA effects ceramide-induced TXNIP expression and NLRP3 inflammasome activation. HUVECs were incubated with TXNIP siRNA for 24 h, followed by 20 μM ceramide for 12 h. Effects of small interfering RNA on the expression of TXNIP (A-C). Effects of si-TXNIP on ceramide-induced pyroptosis-related proteins: NLRP3 (E); TXNIP (F); GSDMD (G); GSDMD-NT (H); Caspase-1 (I); Casp1 p20 (J). Values were reported as the mean ± standard deviation in (A–J) (n = 3). The expression of β-actin was used as an internal control. *P < 0.05, **P < 0.01 and ***P < 0.001 vs control group. ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs ceramide treatment group