Fig. 8

ROS generation mediates ceramide-induced HUVECs’ pyroptosis. HUVECs were treated with various concentrations of C8-ceramide for 12 h, followed by treatment with DCFH-DA for 20 min. A The levels of ROS fluorescence were detected by flow cytometry. B Measurement of ROS fluorescence intensity using a microplate reader. HUVECs were pre-treated with NAC (10 mM) for 1 h, followed by treatment with 20 μM C8-Cer for 12 h and incubation with DCFH-DA for 20 min. C The levels of ROS fluorescence were detected by flow cytometry. D Measurement of ROS fluorescence intensity using a microplate reader. E-F The levels of IL-1β (E) and IL-18 (F) in the supernatants were analysed by ELISA. Values were reported as the mean ± standard deviation in (A–F) (n = 3). ^*P < 0.05, **P < 0.01 and ***P < 0.001 vs control group. ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs ceramide treatment group