Fig. 4

Both SIRT5 and miR-455 are targets of circ-ATL1. A BioEdit software was employed to identify potential miR-455 binding sites within circ-ATL1. The MUT circ-ATL1 form is shown. B Relative luciferase activity was detected 2 days after VSMCs were transfected with miR-455 mimic/normal control (NC) or circ-Astn1 WT/MUT. Results are presented as mean ± SD. ^***P < 0.01. n = 3. C Prediction of miR-455 binding sites within SIRT5 3′-UTR. The 3′-UTR-SIRT5 MUT form is shown. D Relative luciferase activity was detected 2 days after VSMCs were transfected with miR-455 mimic/NC or 3′-UTR-SIRT5 WT/MUT. Results are denoted by mean ± SD. **P < 0.01. n = 3. E-G circ-ATL1 (E), miR-455 (F), and SIRT51 (G) expression levels were measured by RT-qPCR. Results are presented as mean ± SD. ^***P < 0.001 vs. NC. ^###P < 0.001 vs. si-circ-ATL1. n = 3