Table 4 Sequences of the real-time PCR primers
Gene | Primer sequences | Product length (bp) |
|---|---|---|
GSK-3β | Forward: 5′-TGGGTCATTTGGTGTGG − 3′ Reverse: 5′-CCGCAATCGGACTATGTT-3’ | 148 bp |
β-catenin | Forward: 5’- TATGAGTGGGAGCAAGGC − 3′ Reverse: 5′- CTGCGTGGATGGGATCT − 3’ | 150 bp |
cyclin D1 | Forward: 5’- GCGTACCCTGACACCAAT-3′ Reverse: 5′-CTTCGCACTTCTGCTCCT − 3’ | 178 bp |
CTNNBL1 | Forward: 5’- AGGTGGTCGCACTATTGG-3′ Reverse: 5′-GCACATCTCTGGACGGA-3’ | 125 bp |
Gapdh | Forward: 5’- CAAGTTCAACGGCACAGTCAAG − 3′ Reverse: 5′- ACATACTCAGCACCAGCATCAC − 3’ | 123 bp |