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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Cyclic tensile force modifies calvarial osteoblast function via the interplay between ERK1/2 and STAT3

Fig. 3

The osteoblast differentiation and mineralization of primary osteoblasts after ERK1/2 inhibition in the presence or absence of tensile loading. Osteoblasts were treated with U0126 for 12 h, followed by mechanical loading of 24 h. (A) The mRNA expressions of COL-I, ALP, and Runx2 were detected by qPCR. (B) Western blots analysis of the osteogenic markers COL-I, ALP, and Runx2. (C) Alizarin red staining showed the formation of mineralized nodules of osteoblasts after ERK1/2 inhibition and (D) ALP activity (scale bar: 200 μm). Bars represent mean ± SD. *p < 0.05

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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