Using RNA-seq to identify suitable housekeeping genes for hypoxia studies in human adipose-derived stem cells

Table 3 qRT-PCR analysis for selected housekeeping gene candidates in hypoxic and normoxic hADSC cultures

hADSC	Repeats	ALAS1		RRP1		18S		PGK1		GUSB
hADSC	Repeats	Hx	Nx	Hx	Nx	Hx	Nx	Hx	Nx	Hx	Nx
A	1	27.2	25.0	29.1	29.2	21.9	20.3	21.5	25.4
		27.8	24.9	29.7	29.6	21.7	20.1	21.5	25.4
		28.0	25.1	29.9	29.6	21.5	20.2	21.7	25.6
	2	29.6	26.3	29.8	30.5					> 35	27.5
		29.5	26.1	29.6	30.4					> 35	27.3
		29.9	26.2	29.5	30.5					> 35	27.3
B	1	24.9	23.9	25.6	25.7	18.7	20.7	20.1	22.0
		25.0	23.9	25.6	25.7	18.6	20.6	20.1	22.0
		25.0	24.0	25.8	26.1	18.8	20.5	20.1	21.9
	2	29.2	28.4	30.8	29.8					> 35	> 35
		28.9	28.1	30.5	29.7					> 35	> 35
		29.1	28.0	30.8	29.7					> 35	> 35
C	1	26.5	25.9	31.3	31.5	19.5	19.4	22.4	25.7	27.1	26.3
		26.3	25.8	31.1	31.2	19.7	19.3	22.4	25.7	27.1	26.3
		26.4	25.7	31.1	31.1	19.6	19.3	22.4	25.9	27.2	26.2
	2	29.7	29.1	29.9	29.5	20.9	20.9	22.2	25.5	31.8	33.7
		29.5	29.2	29.7	29.3	20.2	20.6	22.4	25.7	31.2	34.2
		29.6	29.0	30.0	29.2	20.3	20.4	22.4	25.7	32.3	> 35
D	1	31.6	29.0	33.6	32.5	26.4	25.2	25.5	29.7	> 35	30.7
		31.8	28.9	33.8	32.6	26.3	25.1	25.5	29.8	> 35	31.0
		31.8	29.0	33.5	32.3	26.5	25.3	25.5	29.4	> 35	30.8

Raw Ct values (n = 4 cell lines, technical replicates n = 3) of ALAS1, RRP1, 18S, PGK1 and GUSB in hADSC-A, -B, -C and -D under normoxic (AN1, AN2, BN1, BN2, CN1, CN2, DN1) and hypoxic (AH1, AH2, BH1, BH2, CH1, CH2, DH1) conditions. In samples where Ct values were 35 or above, gene expression was determined as being below the level of detection and these data were not included

ISSN: 2661-8850