Using RNA-seq to identify suitable housekeeping genes for hypoxia studies in human adipose-derived stem cells

Table 4 Description of TaqMan Assay probes used in qRT-PCR validation of housekeeping genes

Gene	Label	Amplicon (bp)	Assay Design	Catalogue No	Reference
ALAS1	FAM	77	Spanning Exon 9—10	Hs00167441_m1	NM_000688.5
GUSB	FAM	96	Spanning Exon 8—9	Hs00939627_m1	NM_000181.3
POLR2B	FAM	58	Spanning Exon 6—7	Hs00946293_m1	NM_000938.2
RRP1	FAM	75	Spanning Exon 4—5	Hs01002953_m1	NM_003683.5
VEGFA	FAM	59	Spanning Exon 3—4	Hs00900055_m1	NM_001025366.2
18S	VIC	187	Within single exon	Hs99999901_s1	X03205.1
PGK1	VIC	75	Spanning Exon 4—5	Hs99999906_m1	NM_000291.3

Information of TaqMan Assay probes used, and the NCBI Reference Sequences for the corresponding genes. FAM: 5(6)-carboxy-fluorescein; VIC: 2’-chloro-7’phenyl-1,4-dichloro-6-carboxy-fluorescein

ISSN: 2661-8850