Human ASPM participates in spindle organisation, spindle orientation and cytokinesis

Table 2 Effect of siRNA mediated ASPM depletion on spindle pole position and cytokinesis.

	Total number of divisions	Symmetric division with cytokinesis (% of total)	Symmetric division with cytokinesis failure (% of total)	Asymmetric division with cytokinesis (% of total)	Asymmetric division with cytokinesis failure (% of total)
GL3	126	109 (86.5%)	4 (3.2%)	8 (6.3%)	5 (4.0%)
ASP1	94	52 (55.3%)	6 (6.4%)	9 (9.6%)	27 (28.7%)

Time-lapse imaging of living U2OS cells was performed 72 hours post-GL3 and ASP1 siRNA treatment. The results of four independent experiments were combined to produce the final data set. Depletion of ASPM had a profound effect on U2OS cell division, resulting in an increase in the proportion of divisions with the spindle positioned perpendicular to the plate surface (asymmetrical division) and an increase in cytokinesis failure.

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