Figure 8

Thd14 concentrates inside the macronucleus immediately prior to pycnosis. a Fluorescence microscopy images of live conjugating pairs showing localization of GFP-Thd14 at three points in conjugation designated "early" (before meiosis), "mid" (after meiosis, mid-development of anlagen) and "late" (after pair separation, prior to macronuclear reabsorption). M, macronucleus; An, anlagen; m, micronucleus. Live cells were concentrated and mounted in 2% methylcellulose. b DIC and fluorescence microscopy images of a live conjugating pair showing GFP concentration over condensed chromatin regions in degrading macronuclei (arrows). Cells were prepared as in part a.