Figure 4

BMP2 and FGF2 were upregulated through activation of p38 MAPK. Inhibiton of p38 MAPK resulted in the supression of BMP2 and FGF2 transcripts upregulated by BSO treatment in hADMPCs. hADMPCs were pre-treated with 10 μM of SB203580, 10 μM of U0126 or 10 μM of JNK inhibitor II for 2 h followed by 1 mM BSO treatment for 16 h. The medium was replaced with fresh culture medium and the cells were cultured for another 2 days. (A) Western blot analysis of p38 MAPK activation in hADMPCs. (B) Western blot analysis of ERK1/2 MAPK, JNK SAPK activation in hADMPCs. (C, D) Transcription levels of BMP2 (C) and FGF2 (D) were analyzed by q-PCR. The most reliable internal control gene was determined using the geNorm Software.