Immunofluorescence staining for osteocalcin in tissue sections of murine cortical bone and in ceramics seeded with iPSC-TGF-beta1-RA-cells. (A) DAPI staining in a tissue section from a murine cortical bone (B) Immunofluorescence staining for osteocalcin in tissue section of cortical bone (C) Immunofluorescence staining in a tissue section in which primary antibody was omitted. (D) DAPI staining in a tissue section from a ceramic seeded with iPSC-TGF-beta1-RA-cells; (E) Immunofluorescence staining of osteocalcin in a tissue section of a ceramic scaffold seeded with iPSC-TGF-beta1-RA-cells; (F) Overlay image of D and E showing osteocalcin expressing cells colocalizing with DAPI staining cells. Original magnifications 200X. The data confirm that iPSC-cells derived by TGF-beta1 in combination with RA differentiated into osteoblats in vivo and deposited bone.