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Figure 6 | BMC Cell Biology

Figure 6

From: Cells derived from murine induced pluripotent stem cells (iPSC) by treatment with members of TGF-beta family give rise to osteoblasts differentiation and form bone in vivo

Figure 6

Immunofluorescence staining for DMP1 in tissue sections of ceramic scaffolds seeded with iPSC-TGF-beta1-RA-cells and cortical bone. (A) DAPI staining in a tissue section from a murine cortical bone; (B) Immunofluorescence staining for DMP1 in a tissue section of cortical bone; (C) Immunofluorescence staining in a tissue section in which primary antibody was omitted; (D) DAPI staining in a tissue section from a ceramic scaffold seeded with iPSC-TGF-beta1-RA-cells; (E) Immunofluorescence staining of DMP1 in a tissue section of a ceramic scaffold seeded with iPSC-TGF-beta1-RA-cells; (F) Overlay image of D and E indicating that DMP1 expressing cells colocalize with DAPI staining cells. Original magnifications 200X. The data confirm that iPSC-TGF-beta1-RA-cells proceed to osteocyte differentiation in ceramic scaffolds.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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