Skip to main content
Figure 1 | BMC Cell Biology

Figure 1

From: Polycomb group protein expression during differentiation of human embryonic stem cells into pancreatic lineage in vitro

Figure 1

Adaptation of KIND1 cells to feeder free culture system & characterization of feeder free KIND1 cells & embryoid body (EB) differentiation. (A) Bright field images of KIND1 cells cultured on HFF (a) and geltrex (b) Magnification 10X. (c) Cytogenetic analysis of KIND1 exhibiting normal 46, XX chromosome complement. (B) RT-PCR analysis of pluripotency specific gene transcripts (C) Western Blot analysis for OCT4 in undifferentiated feeder free KIND1 cell lysate loaded in triplicate with β actin as loading control. (D) Expression of representative gene transcripts of ectoderm (MAP2), mesoderm (HAND1) and endoderm (MIXL) in Day 7 and Day 15 EBs grown in suspension culture. The expression is with respect to undifferentiated KIND1 cells (set as 1). Error bars represent ± Standard Error of Mean (SEM).

Back to article page

BMC Molecular and Cell Biology

ISSN: 2661-8850

Contact us