Table 1 List of all the primer sequences used for qRT-PCR analysis
Gene | Primer sequence 5′- 3′ | Size |
|---|---|---|
18S rRNA | F- GGAGAGGGAGCCTGAGAAAC | 171Â bp |
R - CCTCCAATGGATCCTCGTTA | ||
BMI1 | F - ACGATGCCCAGCAGCAATGACT | 172Â bp |
R- AAGTGGACCATTCCTTCTCCAGGT | ||
CBX2 | F- GAGTACCTGGTCAAGTGGCG | 215Â bp |
R- TCGGGTTCCTTGAGCTTGGA | ||
CERBERUS | F - GTGCCCTTCAGCCAGACTATAACC | 107Â bp |
R - TGCGCGGCTCCAGGAAAATG | ||
CXCR4 | F - GGCAGCAGGTAGCAAAGTGACGC | 334Â bp |
R - AGAGGAGGTCGGCCACTGACA | ||
E CADHERIN | F- CACTGGGCTGGACCGAGAGAGTT | 236Â bp |
R- ACGCTGGGGTATTGGGGGCA | ||
EED | F – TGCCATTGTGTGCTGGAAACCTGG | 163 bp |
R - GCCCAATGCAAGCATCTTTTGCC | ||
EOMESODERMIN | F - TTCTGGCTTCCGTGCCCACG | 103Â bp |
R - CATGCGCCTGCCCTGTTTCGTA | ||
EZH2 | F- ACACGGGGATAGAGAATGTGGGTT | 455Â bp |
R -TCCGCTTATAAGTGTTGGGTGTTGC | ||
FOXA2 | F - AGGAGGAAAACGGGAAAGAA | 134Â bp |
R - CAACAACAGCAATGGAGGAG | ||
HAND1 | F- TGCCTGAGAAAGAGAACCAG | 273Â bp |
R- ATGGCAGGATGAACAAACAC | ||
HNF4A | F - CCCAGCCCCCTAAGAGAGCAC | 245Â bp |
R - CCCAGCCCCCTAAGAGAGCAC | ||
JARID2 | F – TGCACAAGCCGCAGGACTCG | 356 bp |
R - ACAGCCCCCTATGAGCGGGAG | ||
MAP2 | F- GCATGAGCTCTTGGCAGG | 192Â bp |
R - CCAATTGAACCCATGTAAAGCC | ||
MIXL | F - AGTCCAGGATCCAGGTATGGTTCC | 192Â bp |
R- CAACCCCGTTTGGTTCGGGC | ||
N CADHERIN | F - AACTGGGCCAGGAGCTGACCA | 122Â bp |
R- GTGCCCTCAAATGAAACCGGGCT | ||
NANOG | F - AGTCCCAAAGGCAAACAACCCACTTC | 164Â bp |
R- TGCTGGAGGCTGAGGTATTTCTGTCTC | ||
NKX6.1 | F- CAGAGAGTCAGGTCAAGGTCTGGT | 260Â bp |
R– CTCGGACGCGTGCAGTAGGA | ||
OCT4A | F - AGCCCTCATTTCACCAGGCC | 448Â bp |
R- TGGGACTCCTCCGGGTTTTG | ||
P19 | F- TTGCAGGCCGCCAGTG | 289Â bp |
R- GGGTGTCCAGGAATCCAGTG | ||
PDX1 | F- GAAAGGCCAGTGGGCAGGCGG | 137Â bp |
R- GGCGCGGCCGTGAGATGTAC | ||
REX1 | F- GGCAAACCCACCCCACTCACC | 146Â bp |
R- CAAACACCTGCTGGACTGTGAGC | ||
RING1A | F- AGCCCTACGGAGCGGGAACA | 258Â bp |
R- GGTATCGGCCGCCTCACACG | ||
RING1B | F- CCATGAACAGACTGCAGCGA | 125Â bp |
R- ACTAGGGCCTGCTTCCTGAT | ||
SOX17 | F - AAGGGCGAGTCCCGTATC | 221Â bp |
R- TTGTAGTTGGGGTGGTCCTG | ||
SOX2 | F- GGGGGAAAGTAGTTTGCTGCCTCT | 135Â bp |
R- TGCCGCCGCCGATGATTGTT | ||
SOX9 | F- GACGCTGGGCAAGCTCTGGAGACTT | 141Â bp |
R- TTCTTCACCGACTTCCTCCGCCG | ||
SUZ12 | F- CTGTGGAGGGGGTGGCAGTTAC | 144Â bp |
R- AGGCCTGGAGGAAAAGCTCGTG |