Figure 4

FRET studies to determine interaction between plin5 and the ATGL protein on lipid droplet in adipocytes[39]. Plin5 (1) and ATGL (2) proteins were tagged with FRET probes CFP (3) and YFP (4), respectively. The emission spectra of CFP act as excitation wavelength for the YFP. Detection of yellow fluorescence after activation of CFP (436 nm) suggest possible interaction of the two tagged proteins.