Novel Cul3 binding proteins function to remodel E3 ligase complexes

Table 1 Summary of TAP tag experiments to find Cul3 and actin-binding proteins

Exp. #	# of cells	Total protein (mg)	TAP yield (mg)	Separation	MS input (mg)	Peptides	Peptides </=20 ppm error
Actin-binding proteins bound to 3XFLAG-TEV-CBP-Actin:
1	9.5 × 10⁷	264	64	MudPIT	15	5964	883
Cul3-binding proteins bound to 3XFLAG-TEV-CBP-Cul3:
1	6.9 × 10⁷	192	280	MudPIT	15	4158	562
2	6.9 × 10⁷	192	52	MudPIT	15	1395	234
3	7.5 × 10⁷	208	36	MudPIT	15	1862	393
4	6.5 × 10⁷	180	39	MudPIT	15	1405	276
5	6.5 × 10⁷	180	39	MudPIT	15	1405	117
			Total Cul3 bound peptides:			10225	1582

ISSN: 2661-8850