- Research article
- Open Access
Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee
BMC Cell Biology volume 9, Article number: 20 (2008)
Chimpanzee dental pulp stem/stromal cells (ChDPSCs) are very similar to human bone marrow derived mesenchymal stem/stromal cells (hBMSCs) as demonstrated by the expression pattern of cell surface markers and their multipotent differentiation capability.
ChDPSCs were isolated from an incisor and a canine of a forty-seven year old female chimpanzee. A homogenous population of ChDPSCs was established in early culture at a high proliferation rate and verified by the expression pattern of thirteen cell surface markers. The ChDPSCs are multipotent and were capable of differentiating into osteogenic, adipogenic and chondrogenic lineages under appropriate in vitro culture conditions. ChDPSCs also express stem cell (Sox-2, Nanog, Rex-1, Oct-4) and osteogenic (Osteonectin, osteocalcin, osteopontin) markers, which is comparable to reported results of rhesus monkey BMSCs (rBMSCs), hBMSCs and hDPSCs. Although ChDPSCs vigorously proliferated during the initial phase and gradually decreased in subsequent passages, the telomere length indicated that telomerase activity was not significantly reduced.
These results demonstrate that ChDPSCs can be efficiently isolated from post-mortem teeth of adult chimpanzees and are multipotent. Due to the almost identical genome composition of humans and chimpanzees, there is an emergent need for defining the new role of chimpanzee modeling in comparative medicine. Teeth are easy to recover at necropsy and easy to preserve prior to the retrieval of dental pulp for stem/stromal cells isolation. Therefore, the establishment of ChDPSCs would preserve and maximize the applications of such a unique and invaluable animal model, and could advance the understanding of cellular functions and differentiation control of adult stem cells in higher primates.
Human DPSCs (hDPSCs) were first reported in 2000  and were categorized as post-natal stem cells. DPSCs are capable of differentiating into osteogenic lineages with functions similar to those of osteoblasts [1–3]. A recent study demonstrated the expression of bone markers in DPSCs, including bone sialoprotein, alkaline phosphatase, osteocalcin, and type-I collagen . In addition, like osteoblasts, hDPSCs can differentiate into adipocytes [1, 2]. Although the cell therapeutic potential of DPSCs has not been fully investigated, additional evidence of the multipotent differentiation potential of DPSCs has strongly suggested that DPSCs are able to differentiate beyond mineralized tissues [1, 4–6].
The differentiation capacity and multipotency of DPSCs could be greatly enhanced by the selection of a subset of DPSCs based on cell surface marker expression and DNA dyes exclusion [4, 6]. DPSCs and BMSCs have similar gene expression profiles, although their ability to differentiate varies . Recent discoveries suggest that BMSCs are capable of stimulating neurogenesis through the release of growth factors and the induction of local repair responses [8, 9]. Furthermore, BMSCs have also been considered to be a potential treatment for tumors [10, 11], neurodegenerative diseases [12, 13], and diabetes mellitus . Although there is evidence supporting the notion that BMSCs have potential therapeutic applications, it is yet to be determined whether DPSCs also have a therapeutic function.
In addition to their 98% genetic similarity to humans [15, 16], chimpanzees also show similar cognitive and behavioral characteristics [17–21]. These similarities are sufficient to suggest a critical role for the chimpanzee modeling of human medical conditions, such as Autoimmune Deficiency Syndrome (AIDS), Alzheimer's disease (AD), cancer, malaria, and perimenopausal complications [16, 22–24]. Although mouse, rat, fish and other animal models for these conditions have been developed, the differences in physiology, genetics and pathology from humans make it difficult to extrapolate findings to significant breakthroughs in human medicine [16, 25–27]. Due to the unique genetic and physiologic homology of chimpanzees to humans [16, 28], chimpanzees have been widely used in studies of hepatitis B and C viruses [29–33], AIDS [34, 35], reproduction [36–38] and behavioral development [18–20, 39–41]. However, a chimpanzee model for stem cell research has yet to be developed.
Stem cell research in chimpanzees has not been at the forefront of the field due to limited access, limited availability and highly restricted usage of chimpanzees in research. As delineated above, the unique characteristics of chimpanzees, including a high genomic proximity and close pylogenetic relation with humans, indicate that chimpanzee stem cells could lead to insights into the regulatory mechanisms of gene regulation and differentiation control in humans. There has been much interest in embryonic stem (ES) cells, which have a well-defined differentiation capacity; human, monkey and rodent models have provided an enormous amount of information for comparative studies. However, the establishment of chimpanzee ES cells is not an easy task. DPSCs are easier to obtain than ES cells, however there is much skepticism as to whether or not DPSCs are true pluripotent cells that can mature into fully functional cells from each germ layer [1, 4–6]. Similar to BMSCs, DPSCs are considered as a subgroup of MSCs with different differentiation capability. Although BMSCs could also be recovered during necropsy, DPSCs are alternative source of MSCs with distinctive features that make it a unique and valuable comparative model for elucidating the origin and characteristics of MSCs and ASCs. The recent changes in chimpanzee policy have further reduced the accessibility of chimpanzees in biomedical research. Unlike other animal models, such as monkeys and mice, chimpanzees provide a unique model for the potential discovery of minute differences resulting from the speciation of gene function in humans . Thus, the scientific role of chimpanzees in biomedicine needs to be redefined, and a new venue of chimpanzee modeling is necessary in order to preserve this unique and important model for comparative medicine.
The present study is intended to develop an alternative strategy to preserve and maximize the potential applications of a chimpanzee model for adult stem cell research. Our goal is to evaluate the feasibility of establishing ChDPSCs from post-mortem tissues and determine their multipotent differentiation capacity through direct comparison with hBMSCs and cell surface antigen profiling. We aim to develop ChDPSCs and determine if they can be used as an alternative source of stem cells and serve a novel comparative model in adult stem cell research, which may lead to an increased understanding of stem cell properties and differentiation control in higher primates.
Isolation and culture of ChDPSCs
An incisor and a canine tooth of a forty-seven year old female chimpanzee, euthanized for clinical reasons at the Yerkes National Primate Research Center, were recovered during necropsy. The teeth were stored in DPBS on ice, and delivered to the laboratory. The teeth surfaces were cleaned and dissected at the cementum-enamel junction to reveal the pulp chamber. The dental pulp was recovered followed by digestion in 3 mg/ml collagenase type I (Invitrogen, Inc.) and 4 mg/ml of dispase (Invitrogen, Inc) for one hour at 37°C . The cell suspension was filtered through a 70 μm cell strainer (Falcon, Inc) to recover the single-cell suspensions. The single-cell suspensions of the dental pulp were then cultured in a DPSC medium (α-MEM) supplemented with 20% fetal bovine serum (Hyclone, Inc), 100 μm L-ascorbic acid-2-phosphate (Sigma, Inc), 2 mM glutamine, 100 units/ml penicillin and 100 μg/ml streptomycin (Invitrogen, Inc.), and incubated at 37°C with 5%CO2. Medium was replaced every three to four days and cells were passaged at 70% confluence.
Culture of hBMSCs
hBMSCs were acquired from the Tulane University Health Sciences Center. In brief, hBMSCs were cultured in α-MEM (GIBCO/BRL, Grand Island, NY) supplemented with 20% fetal bovine serum (Atlanta Biologicals, Inc), 100 units/ml penicillin (Invitrogen, Inc.), 100 μg/ml streptomycin (Invitrogen, Inc.), and 2 mM L-glutamine (Invitrogen, Inc.). Cells were then cultured at 37°C with 5% humidified CO2. The hBMSCs were passaged and maintained at low density. hBMSCs were re-plated at 50 cells/cm2 and were harvested at 70% confluence.
Proliferation rate of ChDPSCs
The proliferation rate of ChDPSCs was determined by cell count and MTT assay (ATCC), which was performed every five passages (P5, P10 and P15). For cell count: A total of 5 × 104 cells were plated in a 35 mm dish and a total of three replicas for each cell line were prepared at P5, P10 and P15. At 96 hours post-seeding, ChDPSCs were harvested and a total cell count was determined. For MTT cell proliferation assay: Cell proliferation was determined by using the ATCC MTT Cell Proliferation Assay Kit. A total of 5% or 10% of the ChDPSCs sample from the cell count study was seeded in triplicates in a 96 well plate and cultured overnight. A serially diluted 293FT cell of known number was plated in the same 96 well plate in order to generate a standard curve for later normalization. Each well was brought up to 100 μl total volume and cultured overnight. On the following day, all ChDPSC wells were replaced with 100 μl of fresh culture medium and 10 μl of the MTT reagent was added to each sample and the blank control. After four hours incubation, 100 μl of detergent was added to each well, the plate was covered and kept in the dark at room temperature overnight. Plates were measured with an ELISA reader using an absorbance wavelength of 570 nm on the following day. The readings of the ChDPSCs were normalized by a standard curve based on the 293FT cell and compared between different passages.
Telomere length assay
To determine telomere length, the TeloTAGGG Telomere Length Assay kit from Roche Applied Science was used. In brief, genomic DNA was digested with a mixture of Hinf I and Rsa I restriction enzymes. Digested DNA was then separated in 0.8% agarose gel followed by depurination, denaturation, and neutralization, transferred onto a positively charged nylon membrane. The membrane was hybridized with a DIG labeled telomere probe at 42°C. Three hours later, the membrane was washed in a high stringency buffer and incubated with Anti-DIG-AP solution. After the final wash, AP substrate was applied and exposed on X-ray film.
Colony forming unit (CFU)
Colony forming unit was determined by plating 30 cells/35 mm culture dish followed by 2 weeks culture in a DPSC medium. Medium was replaced every three to four days. After 14 days in culture, the cells were fixed in 100% methanol for 20 minutes followed by 3% crystal violet staining. Any colonies larger than 2 mm in diameter were counted. The CFU equals the total number of colonies divided by the initial number of cells multiplied by 100 as a percentage.
Adipogenic, osteogenic and chondrogenic differentiation
For adipogenic differentiation, the cells were seeded at 400 cells per 35 mm tissue culture dish and cultured for 11 days in DPSC medium. On day 11, the DPSC medium was supplemented with 5.0 μg/ml insulin, 50 μM indomethacin, 1 μM dexamethasone, and 0.5 μM IBMX, which was subsequently replaced every three to four days for three weeks. The culture was then fixed in 4% paraformaldehyde (PFA) followed by Oil-Red-O stain.
For osteogenic differentiation, the cells were prepared as described for adipogenic differentiation until day 11. On day 11, the DPSC medium was supplemented with 1 nM dexamethasone, 50 uM L-Ascorbic acid 2-phosphate sesquimagnesium salt, 20 mM β-glycerolphosphate, and 50 ng/ml L-thyroxine sodium pentahydrate, and was subsequently replaced every three to four days for three weeks. The culture was then fixed in 4% PFA followed by Alizarin Red S stain.
For chondrogenic differentiation, 2.5 × 105 ChDPSCs were centrifuged in a 15 ml conical tube at 1,000 rpm for five minutes. The pellet was maintained in a DPSC medium supplemented with ITS-plus premix (BD Biosciences) to a final concentration of 6.25 ug/ml insulin, 6.25 ug/ml transferrin, and 6.25 ng/ml selenious acid. Additionally, 5.35 ug/ml linoleic acid, 1.25 mg/ml bovine serum albumin, 50 μg/ml Ascorbate 2-phosphate, 40 μg/ml L-proline, 100 μg/ml Sodium pyruvate, 100 nM Dexamethasone, 100 units/ml penicillin, 100 μg/ml streptomycin and 10 ng/ml TGF-β3 (R&D Systems) were also supplemented. Medium was replaced every three to four days for four weeks. The pellets were then fixed in 4% PFA overnight, and the paraffin-embedded sections (4–5 μm) were stained with Alcian blue.
RNA from cell samples of ChDPSCs were prepared with RNeasy Mini kit (Qiagen) for RNA purification and RNase-Free DNase set (Qiagen) for the removal of genomic DNA in the RNA sample. 1.5 ug of total RNA were used to synthesize cDNA by SuperScript III Reverse transcriptase (invitrogen). PCR was performed using a specific primer set targeting stem cell markers: Oct 4 (Oct4-F: 5'- GCA ACC TGG AGA ATT TGT TCC T-3' and Oct4-R: 5'- AGA CCC AGC AGC CTC AAA ATC -3' with an amplicon of 182 bp), Rex-1 (Rex1-F: 5'- GTG AAC AGA ACA GAA GAG GCC -3' and Rex1-R: 5'- GAA ATC GTC CTC TCC AAC AGC -3' with an amplicon of 350 bp), Sox-2 (Sox2-F: 5'- GCA GGT TGA CATC GTT GGT AAT -3' and Sox2-R: 5'- CAA CTA CGG AAA ATA AAG GGG G -3' with an amplicon of 179 bp) and Nanog (Nanog-F: 5'- TCT CTC CTC TTC CTT CCT CCA -3' and Nanog-R: 5'- GGA AGA GTA GAG GCT GGG GT -3' with an amplicon of 389 bp), and differentiation markers: osteonectin (osteonectin-F: 5'- ATC TTC TTT CTC CTT TGC CTG G -3' and osteonectin-R: 5'- GCA CAC CTC TCA AAC TCG CC -3' with an amplicon of 323 bp), osteocalcin (osteocalcin-F: 5'- AGG TGC GAA GCC CAG CGG T -3' and osteocalcin-R: 5'- GCC AGC AGA GCG ACA CCC T -3' with an amplicon of 258 bp), osteopontin (osteopontin-F: 5'- CAG TGA TTT GCT TTT GCC TCC T -3' and osteopontin-R: 5'- CAT TCA ACT CCT CGC TTT CCA T -3' with an amplicon of 507 bp). Bone sialoprotein (BSL-F: 5'- CAC TGG GCT ATG GAG AGG AC -3' and BSL-R: 5'- GCC CTT GCC CTG CCC TCC -3' with an amplicon of 338 bp). GAPDH (GAPDH-F: 5'- ATG GGG AAG GTG AAG GTC GG -3' and GAPDH-R: 5'- CCA TCA CGC CAC AGT TTC CC -3' with an amplicon of 596 bp).
Flow cytometry analysis
Cell lines were divided into 15 FACS tubes (BD Biosciences) at 2 × 105 cells/tube and stained with FITC or PE-conjugated anti-CD14, -CD45, -CD59, -CD73, -CD90, -CD150, -CD166, -IgG1k, -IgG2ak (all from BD Pharmingen), or anti-CD18, -CD24, -CD29, -CD34, -CD44 (all from BD Biosciences), or anti-CD105 (eBioscience). After incubating 20 minutes at ambient temperature in the dark, cells were washed with 2 mL FACS wash solution (dPBS+1%BSA+0.1%NaN3) and centrifuged 5 minutes at 230 × g. Supernatant was removed and cells were fixed with 1% formaldehyde (in dPBS). All data was acquired using a FACS Calibur (Becton Dickinson) and analyzed using CellQuest (Becton Dickinson) and Flowjo software (Treestar, Inc.).
Data analyses were carried out by Student t-test.
Isolation and proliferation rate of ChDPSCs
DPSCs have previously been isolated from humans and other species, including mice and pigs [1, 2, 4]. Here we reported the isolation of ChDPSCs from post-mortem teeth of a forty-seven year old female chimpanzee. The chimpanzee is one of the closest relatives to humans, and ChDPSCs could serve as a powerful comparative model for the study of stemness and differentiation control of adult stem cells in higher primates. The teeth were retrieved from a chimpanzee euthanized at the Yerkes National Primate Research Center (YNPRC), acquired through the tissue distribution program. ChDPSCs share similar morphology to hBMSCs, which are long and spindle shaped (Figure 1a). Two ChDPSC lines were established from the dental pulp of an incisor (ChDPSC-1) and a canine (ChDPSC-2). Both cell lines were capable of forming colonies, with a colony forming unit of 33% and 31% for ChDPSC-1 and ChDPSC-2, respectively, which suggests their capacity for self-renewal and propagation. ChDPSCs have a high proliferation rate at initial and early passages, which decreases gradually in culture (Figure 2). ChDPSC-1 had a higher proliferation rate (11.43 ± 0.47 folds) at passage five compared to that of ChDPSC-2 (7.52 ± 0.69 folds). However, the growth rate of ChDPSC-1 decreased significantly by passage 15 (1.37 ± 0.3 folds) whereas the growth rate of ChDPSC-2 (5.13 ± 0.35 folds) reduced more slowly (p < 0.05; Figure 2). The proliferation study was conducted using two different methods, cell count and MTT proliferation assay, which revealed consistent results.
Assessment of telomerase length in ChDPSCs
Telomere length is one of the most commonly used markers in aging studies. Telomere length decreases as cells continue to divide in vitro and is a natural process in aging . In general, long telomeres (10–20 kb) are observed in stem cells or cells that are vigorously proliferated , whereas the length of telomeres in somatic cells is shorter and ranges from 5–15 kb . To determine the length of the telomeres, a commercially prepared assay was used. Longer telomeres were observed in earlier passages (P5), and while the telomere length gradually decreased in later passages (Figure 3), most of the telomeres remained between 10–20 kb in length. This result is consistent with the results of a prior proliferation study, in which a reduced growth rate of ChDPSCs in culture was parallel with a decrease in telomere length.
Expression of stem cell and differentiation markers in ChDPSCs
In order to determine the multipotent differentiation capability of ChDPSCs, the expression of stem cell specific transcription factors was determined. In addition to stem cell markers, the expression of differentiation markers, such as osteonectin, osteocalcin, osteopontin which is expressed in precursor osteoblasts, was also determined. The expression of the stem cell (Nanog, Sox-2, Rex-1 and Oct-4) and differentiation (Osteonectin, osteocalcin, osteopontin) markers was confirmed in both ChDPSC lines (Figure 4) by RT-PCR analysis using specific primer sets. A similar expression pattern was also reported in rBMSCs  and hDPSCs [1, 2]. Our results suggested the stem cell properties of the ChDPSCs, which were further confirmed in the in vitro differentiation study.
Osteogenic, adipogenic and chondrogenic differentiation of ChDPSCs
Two of the main characteristics of stem cells are self-renewal or self-propagation and competency for multipotent differentiation. Differentiation into osteogenic, adipogenic and chondrogenic lineages is considered to be a trademark event of BMSCs  and DPSCs [1, 2, 7]. Both ChDPSC lines demonstrated potential for osteogenic, adipogenic and chondrogenic differentiation. The osteogenic capability of ChDPSCs (Figure 1b) was comparable to that of hBMSCs , hDPSCs  and rBMSCs . Although the adipogenic capability of hDPSCs [1, 2, 7] has not been clearly demonstrated, adipogenic differentiation of ChDPSCs was clearly confirmed by the formation of fat droplets (Figure 1c), which were morphologically comparable to that of hBMSCs. Furthermore, ChDPSCs were also capable of differentiating into chondrogenic lineages (Figure 1d). Here, we have confirmed the multipotent differentiation capability of ChDPSCs and that this capability is comparable to that of hBMSCs and hDPSCs.
Cell surface antigen profile in ChDPSCs
Besides the expression of stem cell specific transcription factors and a multipotent differentiation capability, the expression profile of a total of 13 cell surface antigens in ChDPSCs and hBMSCs was determined and compared (Figure 5). The cell surface antigen profiles of ChDPSCs and hBMSCs were then compared with published profiles of hBMSCs, hDPSCs and rBMSCs. In both ChDPSC lines, more than 99% of the cells expressed CD29, CD44, CD59, CD73, CD90, CD105, CD150 and CD166, common BMSC markers. However, markers specific for hematopoietic cells, including CD14, CD34, and CD45, were not expressed. Additionally, neither CD18 nor CD24 were expressed in either ChDPSCs or hBMSCs. These results are consistent with previously reported phenotypes of hDPSCs , hBMSCs , and rBMSCs . In fact, this study was composed of a larger panel of cell surface antigens, revealing an almost identical profile for ChDPSCs and hBMSCs, including hBMSCs run in parallel as well as a published profile of hBMSCs . Based on the expression and distribution pattern of the cell surface antigens, the homogeneity of the ChDPSC population was clearly revealed and was similar to that of the hBMSCs.
The rationale for the establishment of ChDPSCs may be questioned, as DPSCs have already been established from other species, including humans [1, 2] and pigs [4, 46]. One asset of chimpanzees as a model for humans is the 98% similarity of their genomes [16, 23, 26, 27], making the chimpanzee the closest living relative to humans; it has even been suggested that the chimpanzee should be re-categorized as homo rather than ape. However, the differences between humans and chimpanzees are the most fascinating question that remains unsolved. Due to an almost identical genomic composition, the dissimilarities are most likely not merely a result of the genetic code but due to a difference in regulatory control of specific gene expression that emerged after the evolutionary split of the two species [16, 25–27]. Geographic and environmental pressure has further accelerated the divergence of the two species, resulting in the advanced speciation of selective gene function in humans. [27, 47, 48]. This suggests that the minute differences resulting from the speciation of gene function in humans could be traced only in a genetically close relative, such as the chimpanzee, but not in other more distantly related species, such as monkeys or mice .
One example of the distinctness of the chimpanzee from other NHPs is the sequence homology of the brain's Tau gene, the key component of filamentous tau deposits in human neurodegenerative diseases [23, 25]. The chimpanzee brain tau was 100% identical to that of the human, whereas the gorilla tau was only 99.5% similar, 99% for gibbons, 98% for rhesus monkeys, 88% for mice and 74% for chickens. Additionally, microarray studies on transcriptome in leukocytes and livers suggest that human gene expression patterns are more similar to those of chimpanzees than those of macaques . These studies reflect how the variation in sequence homologies and expression profiles relates to a species' phylogenetic distance from humans. Moreover, the gene expression patterns in the brain demonstrate a unique correlation between chimpanzees and humans that may not be present between humans and other primates [15, 23, 24, 49].
In vitro differentiation of DPSCs to a designated lineage or cell type has been difficult because of the lack of knowledge in the area of differentiation control. Although tremendous effort has been invested in developing strategies for in vitro-guided differentiation of DPSCs, the underlying regulatory mechanisms remain largely unknown. Spontaneous and guided differentiation has enabled differentiation of hDPSCs to various cell types, including adipocytes [1, 2], osteoblasts [1, 2], chondrocytes [1, 2] and neurons . However, the lack of precise differentiation control and limited homogeneity of the resulting cell types suggest the need for alternate approaches, such as a comparative study using DPSCs derived from a closer species that shares a similar evolutionary path to humans, such as the chimpanzee. A comparative study could accelerate the process of identifying specific regulatory controls that lead to a unique differentiation pathway.
DPSCs are considered to be a potential source of adult stem cells (ASCs) because dental pulp is a vascular connective tissue similar to the mesenchymal tissues found in bone marrow, placenta, muscle, and adipocytes [3, 50]. BMSCs and DPSCs have similar gene expression patterns (Figure 4) and cell surface antigen profiles (Figure 5) even though they are two distinct populations of precursor cells . Although RT-PCR confirmed the expression of stem cell specific transcription factors, we were not able to detect at protein level by either immunocytochemistry or western blot, We speculated the expression level of such transcription factors is extremely low in ChDPSCs. Our result also suggested whether the expression of such transcription factors is required for the maintenance of somatic stem cell properties, which is consistent to prior study on the role of Oct-4 in maintaining of somatic stem cell property . Moreover, DPSCs and BMSCs also have similar biofunctions, such as suppressing T-cell functions in the modulation of immuno responses . According to the current classification and criteria of BMSCs [45, 52, 53], DPSCs are considered to have a lower differentiation potential, with variations in cell surface marker profiles, compared to that of BMSCs . However, the results of our study demonstrated a highly comparable profile between ChDPSCs and hBMSCs (Figure 5; Table 1). Although BMSCs and DPSCs share some common features, they do have different kinetics and differentiation competences . BMSCs are able to develop into osteoblasts, chondrocytes, adipocytes, myelosupportive fibrous-stroma, and other tissue types; whereas DPSCs are capable of developing into osteoblasts with limited evidence of an adipogenic lineage in humans [1–3]. However, we have demonstrated that ChDPSCs have a strong tendency to differentiate into osteoblasts, adipocytes and chondrocytes (Figure 1). It should also be noted that a side-population of DPSCs has been shown to have a more potent differentiation capacity . It has been suggested that DPSCs may represent a subgroup of MSCs at a different stage of differentiation and are therefore committed to a specific function, depending on the microenvironment in which it resides, thus resulting in phenotypic variations .
It is also interesting to note that there was difference in growth pattern between the two ChDPSC lines. We speculated the diverse growth pattern between Ch-DPSC-1 and -2 may be related to the cell type of origin. The two ChDPSCs were derived from the incisor (ChDPSC-1) and the canine (ChDPSC-2) teeth of chimpanzee. The ChDPSC-2 has a more consistent growth rate compared to that of the ChDPSC-1 even they share similar pattern in telomere properties and surface antigen profile. We speculated that canine teeth in species such as chimpanzee continue to grow throughout life and the difference in growth pattern may suggest the difference between teeth type. Thus a comparative study on teeth types merits further investigation.
Compared to bone marrow, dental pulp is easy to access , and proliferates at a higher rate (Figure 2). Although the proliferation rate of ChDPSCs decreased after long term passaging (Figure 2), the telomere length was retained (Figure 3). Homogenous populations of DPSCs can be easily isolated from the dental pulp of post-mortem teeth or those extracted for medical reasons throughout life [1–3]. Based on flow cytometry analysis of the expression profile of cell surface antigens, ChDPSCs presented with high homogeneity (Figure 5; Table 1), which suggests a homogenous population of DPSCs could be established efficiently at early passages.
Furthermore, valuable and endangered species such as chimpanzees, pandas, gorillas, and Siberian tigers, are mostly housed in sanctuaries, research institutions or zoos. Although these species are relatively uncommon in biological studies, they may hold the key to understanding the role of evolution in cell specification and differentiation control. Unfortunately, it is difficult to collect bone marrow, gametes, and other viable tissues from these unique species for stem cell isolation; and it is practically impossible to collect embryonic stem cells (ESCs). Primary cultures from the muscle, the liver, and the brain could be established when tissue is retrieved at necropsy. Unfortunately, the isolation of adult stem cells (ASCs) may not be a practical approach, as most sanctuaries and zoos are not equipped with the tools and skills for emergency recovery of bone marrow or brain tissues for stem cell isolation. Specific preservation and retrieval procedures are often required, and isolation is usually performed immediately upon retrieval in order to have a high recovery yield. Additionally, most ASCs are relatively low in abundance and often difficult to isolate. The exception is the isolation of BMSCs from bone marrow and cord blood where efficient isolation procedures have been established. Based on these reasons, the isolation of ASCs is not possible for most of these species, unless arrangements have been well planned in advance. On the other hand, the isolation of DPSCs from dental pulp is simple and has a high success rate. Most necropsy facilities have the tools for teeth retrieval and no special preservation or handling procedures are required. Teeth could be retrieved, preserved in saline, kept at 4°C, and would then be ready for shipment. In fact, teeth preserved overnight at 4°C have a comparable isolation efficiency compared with fresh dental pulp from monkeys (unpublished data). Therefore, it is possible to recover DPSCs from valuable and endangered species with no geographic limitations. Stem cells from such species are invaluable research materials for studying differentiation control in species with unique habitats and positions on the phylogeny tree.
In conclusion, DPSCs are easy to establish from the dental pulp, capable of self-renewal, and differentiate to multiple lineages. Although DPSCs are primarily considered to have applications in paradontology, implantology, and calcified tissue reconstruction [3, 4, 6, 53, 54], clinical application of DPSCs should not be limited to hard tissue engineering. It seems that DPSCs have a similar, if not identical, potential to BMSCs , and therefore more diversified clinical applications should be investigated. Most importantly, ChDPSCs are valuable as a comparative model in ASC research, which may lead to insights into the understanding of stem cell properties and differentiation control in higher primates, such as humans.
Gronthos S, Mankani M, Brahim J, Robey PG, Shi S: Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proceedings of the National Academy of Sciences of the United States of America. 2000, 97 (25): 13625-13630. 10.1073/pnas.240309797.
Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A, DenBesten P, Robey PG, Shi S: Stem cell properties of human dental pulp stem cells. J Dent Res. 2002, 81 (8): 531-535.
Pierdomenico L, Bonsi L, Calvitti M, Rondelli D, Arpinati M, Chirumbolo G, Becchetti E, Marchionni C, Alviano F, Fossati V, Staffolani N, Franchina M, Grossi A, Bagnara GP: Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp. Transplantation. 2005, 80 (6): 836-842. 10.1097/01.tp.0000173794.72151.88.
Iohara K, Zheng L, Ito M, Tomokiyo A, Matsushita K, Nakashima M: Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis. Stem cells (Dayton, Ohio). 2006, 24 (11): 2493-2503. 10.1634/stemcells.2006-0161.
Laino G, d'Aquino R, Graziano A, Lanza V, Carinci F, Naro F, Pirozzi G, Papaccio G: A new population of human adult dental pulp stem cells: a useful source of living autologous fibrous bone tissue (LAB). J Bone Miner Res. 2005, 20 (8): 1394-1402. 10.1359/JBMR.050325.
Laino G, Graziano A, d'Aquino R, Pirozzi G, Lanza V, Valiante S, De Rosa A, Naro F, Vivarelli E, Papaccio G: An approachable human adult stem cell source for hard-tissue engineering. Journal of cellular physiology. 2006, 206 (3): 693-701. 10.1002/jcp.20526.
Shi S, Robey PG, Gronthos S: Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. Bone. 2001, 29 (6): 532-539. 10.1016/S8756-3282(01)00612-3.
Munoz JR, Stoutenger BR, Robinson AP, Spees JL, Prockop DJ: Human stem/progenitor cells from bone marrow promote neurogenesis of endogenous neural stem cells in the hippocampus of mice. Proceedings of the National Academy of Sciences of the United States of America. 2005, 102 (50): 18171-18176. 10.1073/pnas.0508945102.
Prockop DJ, Gregory CA, Spees JL: One strategy for cell and gene therapy: harnessing the power of adult stem cells to repair tissues. Proceedings of the National Academy of Sciences of the United States of America. 2003, 100 (Suppl 1): 11917-11923. 10.1073/pnas.1834138100.
Nakamizo A, Marini F, Amano T, Khan A, Studeny M, Gumin J, Chen J, Hentschel S, Vecil G, Dembinski J, Andreeff M, Lang FF: Human bone marrow-derived mesenchymal stem cells in the treatment of gliomas. Cancer research. 2005, 65 (8): 3307-3318.
Studeny M, Marini FC, Champlin RE, Zompetta C, Fidler IJ, Andreeff M: Bone marrow-derived mesenchymal stem cells as vehicles for interferon-beta delivery into tumors. Cancer research. 2002, 62 (13): 3603-3608.
Hellmann MA, Panet H, Barhum Y, Melamed E, Offen D: Increased survival and migration of engrafted mesenchymal bone marrow stem cells in 6-hydroxydopamine-lesioned rodents. Neurosci Lett. 2006, 395 (2): 124-128. 10.1016/j.neulet.2005.10.097.
Lu L, Zhao C, Liu Y, Sun X, Duan C, Ji M, Zhao H, Xu Q, Yang H: Therapeutic benefit of TH-engineered mesenchymal stem cells for Parkinson's disease. Brain Res Brain Res Protoc. 2005, 15 (1): 46-51. 10.1016/j.brainresprot.2005.03.002.
Lee RH, Seo MJ, Reger RL, Spees JL, Pulin AA, Olson SD, Prockop DJ: Multipotent stromal cells from human marrow home to and promote repair of pancreatic islets and renal glomeruli in diabetic NOD/scid mice. Proceedings of the National Academy of Sciences of the United States of America. 2006, 103 (46): 17438-17443. 10.1073/pnas.0608249103.
Karaman MW, Houck ML, Chemnick LG, Nagpal S, Chawannakul D, Sudano D, Pike BL, Ho VV, Ryder OA, Hacia JG: Comparative analysis of gene-expression patterns in human and African great ape cultured fibroblasts. Genome research. 2003, 13 (7): 1619-1630. 10.1101/gr.1289803.
Varki A: A chimpanzee genome project is a biomedical imperative. Genome research. 2000, 10 (8): 1065-1070. 10.1101/gr.10.8.1065.
Call J: Chimpanzee social cognition. Trends Cogn Sci. 2001, 5 (9): 388-393. 10.1016/S1364-6613(00)01728-9.
de Waal FB: A century of getting to know the chimpanzee. Nature. 2005, 437 (7055): 56-59. 10.1038/nature03999.
Parr LA: Perceptual biases for multimodal cues in chimpanzee (Pan troglodytes) affect recognition. Animal cognition. 2004, 7 (3): 171-178. 10.1007/s10071-004-0207-1.
Parr LA, Waller BM, Vick SJ, Bard KA: Classifying chimpanzee facial expressions using muscle action. Emotion Washington, DC. 2007, 7 (1): 172-181.
Van Hooff JA: A component analysis of the structure of the social behaviour of a semi-captive chimpanzee group. Experientia. 1970, 26 (5): 549-550. 10.1007/BF01898505.
Brent L: Solutions for research chimpanzees. Lab animal. 2004, 33 (1): 37-43.
Holzer M, Craxton M, Jakes R, Arendt T, Goedert M: Tau gene (MAPT) sequence variation among primates. Gene. 2004, 341: 313-322. 10.1016/j.gene.2004.07.013.
Marvanova M, Menager J, Bezard E, Bontrop RE, Pradier L, Wong G: Microarray analysis of nonhuman primates: validation of experimental models in neurological disorders. Faseb J. 2003, 17 (8): 929-931.
Enard W, Khaitovich P, Klose J, Zollner S, Heissig F, Giavalisco P, Nieselt-Struwe K, Muchmore E, Varki A, Ravid R, Doxiadis GM, Bontrop RE, Paabo S: Intra- and interspecific variation in primate gene expression patterns. Science New York, NY. 2002, 296 (5566): 340-343.
Gu J, Gu X: Induced gene expression in human brain after the split from chimpanzee. Trends Genet. 2003, 19 (2): 63-65. 10.1016/S0168-9525(02)00040-9.
Wildman DE: A map of the common chimpanzee genome. Bioessays. 2002, 24 (6): 490-493. 10.1002/bies.10103.
Muchmore EA: Chimpanzee models for human disease and immunobiology. Immunological reviews. 2001, 183: 86-93. 10.1034/j.1600-065x.2001.1830107.x.
Bukh J: A critical role for the chimpanzee model in the study of hepatitis C. Hepatology Baltimore, Md. 2004, 39 (6): 1469-1475.
Magiorkinis EN, Magiorkinis GN, Paraskevis DN, Hatzakis AE: Re-analysis of a human hepatitis B virus (HBV) isolate from an East African wild born Pan troglodytes schweinfurthii: evidence for interspecies recombination between HBV infecting chimpanzee and human. Gene. 2005, 349: 165-171. 10.1016/j.gene.2004.12.021.
Makuwa M, Souquiere S, Clifford SL, Mouinga-Ondeme A, Bawe-Johnson M, Wickings EJ, Latour S, Simon F, Roques P: Identification of hepatitis B virus genome in faecal sample from wild living chimpanzee (Pan troglodytes troglodytes) in Gabon. J Clin Virol. 2005, 34 (Suppl 1): S83-88. 10.1016/S1386-6532(05)80016-2.
Prince AM, Brotman B, Lee DH, Pfahler W, Tricoche N, Andrus L, Shata MT: Protection against chronic hepatitis C virus infection after rechallenge with homologous, but not heterologous, genotypes in a chimpanzee model. The Journal of infectious diseases. 2005, 192 (10): 1701-1709. 10.1086/496889.
Puig M, Mihalik K, Tilton JC, Williams O, Merchlinsky M, Connors M, Feinstone SM, Major ME: CD4+ immune escape and subsequent T-cell failure following chimpanzee immunization against hepatitis C virus. Hepatology Baltimore, Md. 2006, 44 (3): 736-745.
Wei Q, Fultz PN: Extensive diversification of human immunodeficiency virus type 1 subtype B strains during dual infection of a chimpanzee that progressed to AIDS. Journal of virology. 1998, 72 (4): 3005-3017.
Novembre FJ, Saucier M, Anderson DC, Klumpp SA, O'Neil SP, Brown CR, Hart CE, Guenthner PC, Swenson RB, McClure HM: Development of AIDS in a chimpanzee infected with human immunodeficiency virus type 1. Journal of virology. 1997, 71 (5): 4086-4091.
Gould KG, Johnson-Ward J: Use of intrauterine devices (IUDs) for contraception in the common chimpanzee (Pan troglodytes). Journal of medical primatology. 2000, 29 (2): 63-69. 10.1034/j.1600-0684.2000.290203.x.
Gould KG, Young LG: Acquisition of fertilizing capacity by chimpanzee sperm. Folia primatologica; international journal of primatology. 1990, 54 (1–2): 105-108.
Smithwick EB, Young LG: Histological effects of androgen deprivation on the adult chimpanzee epididymis. Tissue & cell. 2001, 33 (5): 450-461. 10.1054/tice.2001.0199.
Burrows AM, Waller BM, Parr LA, Bonar CJ: Muscles of facial expression in the chimpanzee (Pan troglodytes): descriptive, comparative and phylogenetic contexts. Journal of anatomy. 2006, 208 (2): 153-167. 10.1111/j.1469-7580.2006.00523.x.
Hopkins WD: Chimpanzee right-handedness: internal and external validity in the assessment of hand use. Cortex; a journal devoted to the study of the nervous system and behavior. 2006, 42 (1): 90-93.
Taglialatela JP, Cantalupo C, Hopkins WD: Gesture handedness predicts asymmetry in the chimpanzee inferior frontal gyrus. Neuroreport. 2006, 17 (9): 923-927. 10.1097/01.wnr.0000221835.26093.5e.
Izadpanah R, Joswig T, Tsien F, Dufour J, Kirijan JC, Bunnell BA: Characterization of multipotent mesenchymal stem cells from the bone marrow of rhesus macaques. Stem Cells Dev. 2005, 14 (4): 440-451. 10.1089/scd.2005.14.440.
Allsopp RC, Vaziri H, Patterson C, Goldstein S, Younglai EV, Futcher AB, Greider CW, Harley CB: Telomere length predicts replicative capacity of human fibroblasts. Proceedings of the National Academy of Sciences of the United States of America. 1992, 89 (21): 10114-10118. 10.1073/pnas.89.21.10114.
Martens UM, Zijlmans JM, Poon SS, Dragowska W, Yui J, Chavez EA, Ward RK, Lansdorp PM: Short telomeres on human chromosome 17p. Nature genetics. 1998, 18 (1): 76-80. 10.1038/ng0198-018.
Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop D, Horwitz E: Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006, 8 (4): 315-317. 10.1080/14653240600855905.
Sonoyama W, Liu Y, Fang D, Yamaza T, Seo BM, Zhang C, Liu H, Gronthos S, Wang CY, Shi S, Wong S: Mesenchymal stem cell-mediated functional tooth regeneration in swine. PLoS ONE. 2006, 1: e79-10.1371/journal.pone.0000079.
Hacia JG: Genome of the apes. Trends Genet. 2001, 17 (11): 637-645. 10.1016/S0168-9525(01)02494-5.
Hey J: Speciation and inversions: chimps and humans. Bioessays. 2003, 25 (9): 825-828. 10.1002/bies.10336.
Caceres M, Lachuer J, Zapala MA, Redmond JC, Kudo L, Geschwind DH, Lockhart DJ, Preuss TM, Barlow C: Elevated gene expression levels distinguish human from non-human primate brains. Proceedings of the National Academy of Sciences of the United States of America. 2003, 100 (22): 13030-13035. 10.1073/pnas.2135499100.
Vaananen HK: Mesenchymal stem cells. Annals of medicine. 2005, 37 (7): 469-479. 10.1080/07853890500371957.
Lengner CJ, Camargo FD, Hochedlinger K, Welstead GG, Zaidi S, Gokhale S, Scholer HR, Tomilin A, Jaenisch R: Oct4 expression is not required for mouse somatic stem cell self-renewal. Cell Stem Cell. 2007, 1 (4): 403-415. 10.1016/j.stem.2007.07.020.
Kern S, Eichler H, Stoeve J, Kluter H, Bieback K: Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem cells (Dayton, Ohio). 2006, 24 (5): 1294-1301. 10.1634/stemcells.2005-0342.
Nakashima M, Akamine A: The application of tissue engineering to regeneration of pulp and dentin in endodontics. Journal of endodontics. 2005, 31 (10): 711-718. 10.1097/01.don.0000164138.49923.e5.
Papaccio G, Graziano A, d'Aquino R, Graziano MF, Pirozzi G, Menditti D, De Rosa A, Carinci F, Laino G: Long-term cryopreservation of dental pulp stem cells (SBP-DPSCs) and their differentiated osteoblasts: a cell source for tissue repair. Journal of cellular physiology. 2006, 208 (2): 319-325. 10.1002/jcp.20667.
Chimpanzee teeth were acquired through the tissue distribution program of the Yerkes National Primate Research Center (YNPRC). We thank for the Tulane University Health Sciences Center providing the hBMSCs. Critical review and suggestions provided by Ms. Heather Banta and all at the YNPRC for thoughtful comments and assistance. Yerkes National Primate Research Center is supported by the base grant No.RR-00165 awarded by the Animal Resources Program of the NIH. We also like to acknowledge the Emory Vaccine Center for AIDS Research Immunology Core Laboratory (P30A1050409). AWSC is supported by grant awarded by the NCRR/NIH (RR018827-04).
PHC: ChDPSC culture and characterization. BS: hBMSCs culture and preparation for FACs. DF and CI: FACs analysis. AHCH: experimental design. AWSC: ChDPSC isolation, experimental design and manuscript preparation. All authors read and approved the final manuscript.
About this article
Cite this article
Cheng, P., Snyder, B., Fillos, D. et al. Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee. BMC Cell Biol 9, 20 (2008). https://doi.org/10.1186/1471-2121-9-20
- Telomere Length
- Adult Stem Cell
- Dental Pulp
- Chondrogenic Differentiation
- Adipogenic Differentiation