Skip to main content
Fig. 2 | BMC Cell Biology

Fig. 2

From: Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation

Fig. 2

Myogenic marker expression of isolated SC/MPC populations. Flow cytometric analysis of immunofluorescence-stained SC/MPC from SM and LD muscle cultured for 4 days (a) and 8 days (b) in growth medium. The cells were immunostained for Pax7, MyoD1, MyoG, and Desmin. Percentages of positive cells in each sample are presented as Box-Whisker plots with the median and the maximum 1.5 of the interquartile range (Q1-Q3). Outliers are included in the figure as circles but were excluded from statistical analysis. All populations expressed the selected myogenic markers, whereas a higher proportion of cells were positive for MyoG and Desmin, which are markers for terminally committed myogenic cells. Statistical analysis was performed by ANOVA (with the Holm-Sidak method as a pairwise multiple comparison procedure) or the Kruskal-Wallis One-Way ANOVA on Ranks (with Dunn’s method as a pairwise multiple comparison procedure), *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, n = 4–9 animals

Back to article page
\