Fig. 6
Myotube size distribution and fusion rate of differentiated SC/MPC populations. a, b Quantitative analysis of MHC immunofluorescence images to determine myotube area. a Each point represents the size of a single MHC+ myotube that contains two or more nuclei, and the horizontal line illustrates the mean value. b Myotubes with a size of < 10,000 μm2 were classified as small myotubes, whereas myotubes ≥10,000 μm2 were grouped as middle-sized and large myotubes. c Quantitative analysis of Desmin immunofluorescence images to determine fusion rate. SP40/50 contained more middle-sized and large myotubes, resulting in a significantly higher average myotube area in comparison with mixed P40/70 and SP50/70. Furthermore, significantly higher fusion rates were determined in SP40/50 compared with mixed P40/70 and SP50/70. The results represent three individual experiments from 3 animals. Statistical analysis was performed by a One-Way ANOVA (fusion rate) or Kruskal-Wallis One-Way ANOVA on Ranks (myotube area and distribution) followed by the corresponding pairwise comparison test, and significant differences are marked with asterisks in Fig. 6a and c (**p ≤ 0.01, ***p ≤ 0.001) or letters in fig. 6b (p ≤ 0.05)