Fig. 5
Effects of Tbx2 deletion constructs on the BMP/GDF and Activin/Nodal pathways a) Embryos were injected with 2 ng of tbx2 or tbx2S1T in the animal pole at the two-cell stage. Animal caps were explanted at stage 8.5. Activin was added (2.5 ng/ml) as indicated. b Embryos were injected with 2 ng of tbx2 or tbx2NS1T in the animal pole at the two-cell stage. Animal caps were explanted at stage 8.5. Activin was added (2.5 ng/ml) as indicated. c Embryos were injected with 2 ng of tbx2 or tbx2NS1T in the animal pole at the two-cell stage. Animal caps were explanted at stage 8.5. d Embryos were injected with 1 ng tbx2 or tbx2ΔN in the animal pole at the two-cell stage. Animal caps were explanted at stage 8.5. Activin was added (2.5 ng/ml) as indicated e) Embryos were injected with 1 ng tbx2 or tbx2TS2C in the animal pole at the two-cell stage. Animal caps were explanted at stage 8.5. Activin was added (2.5 ng/ml) as indicated