Fig. 6
From: Association of Sonic Hedgehog with the extracellular matrix requires its zinc-coordination center
Cholesterol-modified ShhNp associates with the ECM in a zinc and peptidase-dependent manner. a In situ staining with anti ShhN mAb5E1 of ECM deposited by Ptch1LacZ/LacZ;Ptch2−/−;Boc−/−;Cdo−/−;Gas1−/− cells that were transfected with Shh or Shh-C199* in the presence or absence of 5 μM zinc and presence of 0.18 mM calcium. b Quantification of ECM-bound Shh shown in A. Box and whisker plots of mean fluorescence intensity per image of 10 microscope fields per experiment was measured in ImageJ and normalized to the ECM of mock transfected cells. n = 3, ****p < 0.0001)