Fig. 4

Wound healing assay. Cells were treated with 100 μl of conditioned media from DU145 cultures that had been transfected by sFLT01containing construct and/or control consteucts. After 24–48 h incubation in humidified CO2 incubator, cultures were fixed in 3% formaldehyde solution and visualized by a light microscope. a, b DU145 cells migration as a negative control. c, d migration of DU145 cells which had been treated by CM from pAAV-MCS-GFP / control cultures. e, f migration in DU145 cultures which had been treated by CM from pAAV-sFLT01-HisTag-GFP transfected cultures. g data indicates that following treatment with sFLT01 protein containing CM for 24 h and 48 h, the mobility of DU145 cells was significantly decreased versus the control group (treated by CM from pAAV-MCS-GFP transfected cells); 0.43-fold (P < 0.05) versus 2.27-fold (P < 0.001)