Fig. 3

Network diagram and simulations of the extended model of HGF-activated RhoA-NO network, including known effects of ‘tension’ on NO and RhoA. A Red arrows represent the inhibitory reactions. Complete specification of the extended model appears in Additional file 1 Tables S1 and S2. B Simulation of the extended model, starting with 100 random levels of all species. Trajectories of the RhoA concentration show convergence to two different steady states. Note faster convergence than in Fig. 2. C and D Bifurcation analysis of the extended model. The steady state NO concentration is plotted against change in parameters C ki1 (rate constant for ROCK inhibition of Akt phosphorylation) and D ki2 (rate constant for RhoA inhibition of eNOS mRNA). Note that bistability occurs over a wider range of ki1 and ki2, compared with Fig. 2. E Robustness analysis (as in Fig. 2) applied to the extended model. White boxes denote the presence of bistability while black boxes denote the absence of bistability. The red line highlights the unperturbed parameter set