Fig. 2

A, Control of gene expression by real-time PCR. Real-time PCR results are consistent with our differential transcriptomic analysis, with however expression level of KH.C7.314 between beginning and mid-tail regression of control larvae not statistically significant. BC: Tail regression beginning, control. BZ: Tail regression beginning, Z-VAD. MC: Mid-tail regression, control. MZ: Mid-tail regression, Z-VAD. * indicates statistically significant differences. B, In situ hybridization of KH.L132.4 (matrilin-2) gene (in green) combined with immunnodection of Ci-VASA to detect PGC (in red) in premetamorphic larva. Matrilin-2 is expressed in epidermal cells at the tip of the tail, the most anterior expressing epidermal cells are in contact with PGC (higher magnification, white square). Matrilin-2 expression is also detected in the trunk epidermis. White square higher magnification of the PGC region