Fig. 4

Alteration of the leucine-rich NES in Swi6 reduces MBF-mediated expression, but not SBF-mediated expression. Yeast containing a swi6Δ deletion were transformed with empty vector (swi6Δ), a centromeric plasmid expressing wild-type SWI6 (SWI6), or a centromeric plasmid expressing full-length SWI6 with each of the leucines in the leucine-rich NES converted to an alanine (swi6ΔNES). Each strain was also transformed with a reporter plasmid containing either an SBF-response element (A) or MBF-response element (B) upstream of the LacZ gene. Cells were grown to log phase, lysed, and extracts analyzed for β-galactosidase activity. Bars represent average of at least three assays for each strain. Error bars show s.e.m. for each. ** = significant difference between indicated paired samples using Student’s t-test (p < 0.005). * = significant difference among the three samples using ANOVA (p < 0.01)